Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Colorimetric Assay Kit (E-BC-K605-M)
For research use only.
| Detection Principle |
Phosphoenolpyruvate Carboxykinase (PEPCK) is a key metabolic enzyme that plays a central role in processes such as gluconeogenesis and triglyceride synthesis. As a central regulator for maintaining blood sugar homeostasis and energy metabolism, PEPCK catalyzes the formation of phosphoenolpyruvate (PEP) from oxaloacetic acid, serving as a critical link between carbohydrate metabolism, lipid metabolism, and amino acid metabolism. The detection principle of this kit: PEPCK catalyzes the substrate reaction and consumes the reducing agent, which has a maximum absorbance at 340 nm. The enzyme activity of PEPCK is calculated by determining the amount of reducing agent consumed per unit time during the reaction. |
| Sample Type | Animal tissue,Plant tissue,Cell,Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader(340 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 9.38 U/L |
| Detection Range | 9.38-231.65 U/L |
| Precision | inter-assay CV: 4.8%-8.3% | intra-assay CV: 4.0%-5.0% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 100-200 |
| 10% Mouse kindey tissue homogenate | 100-200 |
| 10% Mouse spleen tissue homogenate | 50-100 |
| 10% Mouse lung tissue homogenate | 50-100 |
| 10% Corn tissue homogenate | 50-100 |
| 1×10^6 HL-60 cells | 2-5 |
| 1×10^6 293Tcells | 2-5 |
| 1×10^6 Jurkat cells | 2-5 |
| Mouse serum | 1-3 |
| Mouse plasma | 1-3 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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