Protease Activity Colorimetric Assay Kit (E-BC-K886-M)

Name: Protease Activity Colorimetric Assay Kit
Brand: Elabscience
SKU: E-BC-K886-M
Price: 320.0 USD
Availability: InStock

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Protease Activity Colorimetric Assay Kit - 1

All	Size	Price	Qty
	96T	$ 320.00	- +

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Detection Principle	Protease is a class of enzymes capable of catalyzing the hydrolysis of protein peptide bonds and belongs to the hydrolase family. They break down the substrate into smaller peptides or free amino acids by breaking the amide bonds (-CO-NH-) in proteins or peptides. Proteases are widely present in living organisms and are involved in various physiological processes, such as digestion, cell signal transduction, immune response and apoptosis. The detection principle of this kit is: Protease hydrolyzes substrates to generate products. The activity of protease in the sample can be calculated by measuring the absorbance change of the product per unit time at a wavelength of 750 nm.
Sample Type	Animal tissue,Plant tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (730-770 nm, optimum wavelength: 750 nm)
Research Area	Amino Acids And Proteins
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.004 U/L
Detection Range	0.004-0.50 U/L
Precision	inter-assay CV: 3.7%-7.9% \| intra-assay CV: 1.3%-4.4%
Sample Volume	50 μL(tissue homogenate)
Assay Time	1 h

Cat.No.	Product Name	Sizes
E-BC-K843-M	Trypsin Activity Assay Kit	96T , 48T
E-BC-K845-M	Chymotrypsin Activity Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse gastric tissue homogenate	1
10% Rat gastric tissue homogenate	1
10% Mouse intestinal tissue homogenate	1
10% Papaya flesh tissue homogenate	1
10% Pineapple flesh tissue homogenate	1
10% Kiwi fruit flesh tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.