Purified Anti-Human CD74 Antibody[LL1] (E-AB-F14210P)
![Purified Anti-Human CD74 Antibody[LL1] - 1](http://file.elabscience.com/assets/images/loading.png)
For research use only.
| Verified Samples |
Verified Samples in FCM: Human peripheral blood lymphocytes Verified Samples in WB: Raji |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), WB 1:200-1:500 |
| Isotype | Mouse IgG1, κ |
| Host | Mouse |
| Reactivity | Human |
| Applications | FCM, WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CD74 protein |
| Abbre | CD74 |
| Synonyms | DHLAG, CD74, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain (Ii), HLA class II histocompatibility antigen gamma chain |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
30-35 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | ≥ 1 mg/mL |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Purification Method | >98%, Protein A/G purified |
| Research Areas | Immunology |
| Clone No. | LL1 |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CD74 is a type II transmembrane glycoprotein also known as MHC class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, and MIF receptor. CD74 exists in four isoforms with molecular masses of 33, 35, 41, and 43 kD, depending on genetic splicing. CD74 is primarily expressed on antigen presenting cells, including B cells, monocytes/macrophages, dendritic cells, and Langerhans cells. It is also expressed by activated T cells and activated endothelial and epithelial cells as well as carcinomas of lung, renal, gastric and thymic origin. The primary function of CD74 is intracellular sorting of MHC class II molecules and regulation of exogenous peptide loading onto MHC class II. It is also involved in the modulation of B cell differentiation and positive selection of CD4+ T cells. It has been reported that CD74 binds MIF (macrophage migration inhibitory factor) and signals through CD44 to regulate innate and adaptive immunity. It is also reported that H. pylori infection occurs through urease B binding of CD74 on gastric epithelial cells, inducing gastric epithelial cell apoptosis, NF-κB activation, and IL-8 production. |
Other Clones
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Other Formats
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APC
Biotin
Elab Fluor®488
Elab Fluor®647
Elab Fluor®700
FITC
PE
PE/Cyanine 5
PE/Cyanine 5.5
PE/Cyanine 7
PE/Elab Fluor®594
PerCP/Cyanine 5.5
Unconjugated
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