Purified Anti-Human Oct-3/4 Antibody[40/Oct-3] (AN010010P)
| Alternate Names | OCT-3/4, Octamer-4, POU Class 5 Homoeobox 1, POU Domain Class 5 Transcription Factor 1, POU5F, OTF, POU5F1, OTF-3, OTF3, OTF4, Oct-3, Oct-4 |
| Clone | |
| Verified Samples | Verified Samples in FCM: NCCIT cells |
| Dilution | FCM ≤ 0.2 µg per million cells in 100 µL volume |
| Background | Development of a multicellular organism from a single fertilized cell is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem cell (ES) lines and embryonal carcinomas (EC). Other members of the POU family include Oct1, Oct2, Pit-1, and unc-86. The POU domain, a 150-amino acid region that determines binding specificity, is conserved among these proteins and consists of 3 subdomains: POU-specific A and B subdomains and a homeobox-like subdomain. Oct3/4 is expressed in undifferentiated cells, but is lost as cells are induced to differentiate. Oct3/4 is not expressed in adult tissues. The interaction of Oct3/4 with SOX2, another embryonic transcription factor, produces an active complex that regulates expression of genes such as Nanog, UTF1, and FGF4. Although Oct3/4 is specifically phosphorylated on serine residues, this modification is not required for DNA binding, but may affect its transactivation potential. Thus, Oct3/4 is a transcription factor that plays an important role in determining early steps of embryogenesis and differentiation. |
| Immunogen | Recombinant Human Oct-3/4 protein |
| Abbre | Oct-3/4 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG1, κ |
| Isotype Control | Purified Mouse IgG1, κ Isotype Control[MOPC-21] |
| Applications |
FCM
|
| Research Areas | Epigenetics and Nuclear Signaling;Developmental Biology;Stem Cells;Pluripotency Maintenance and Differentiation;Embryonic Stem Cells;Germline Stem Cells Markers |
| Cellular Localization |
Cytoplasm, Nucleus
|
| Tissue Specificity |
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | Unconjugated |
| Storage Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
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