Purified Anti-Human Oct-3/4 Antibody[40/Oct-3] (AN010010P)
![Purified Anti-Human Oct-3/4 Antibody[40/Oct-3] - 1](http://file.elabscience.com/assets/images/loading.png)
Add to cart
For research use only.
| Verified Samples | Verified Samples in FCM: NCCIT cells |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells) |
| Isotype | Mouse IgG1, κ |
| Host | Mouse |
| Reactivity | Human |
| Applications | FCM |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Oct-3/4 protein |
| Abbre | Oct-3/4 |
| Synonyms | OCT-3/4, Octamer-4, POU Class 5 Homoeobox 1, POU Domain Class 5 Transcription Factor 1, POU5F, OTF, POU5F1, OTF-3, OTF3, OTF4, Oct-3, Oct-4 |
| Swissprot | |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Specificity | Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. |
| Concentration | ≥ 1 mg/mL |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Purification Method | >98%, Protein A/G purified |
| Research Areas | Epigenetics and Nuclear Signaling, Developmental Biology, Stem Cells, Pluripotency Maintenance and Differentiation, Embryonic Stem Cells, Germline Stem Cells Markers |
| Clone No. | 40/Oct-3 |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Development of a multicellular organism from a single fertilized cell is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem cell (ES) lines and embryonal carcinomas (EC). Other members of the POU family include Oct1, Oct2, Pit-1, and unc-86. The POU domain, a 150-amino acid region that determines binding specificity, is conserved among these proteins and consists of 3 subdomains: POU-specific A and B subdomains and a homeobox-like subdomain. Oct3/4 is expressed in undifferentiated cells, but is lost as cells are induced to differentiate. Oct3/4 is not expressed in adult tissues. The interaction of Oct3/4 with SOX2, another embryonic transcription factor, produces an active complex that regulates expression of genes such as Nanog, UTF1, and FGF4. Although Oct3/4 is specifically phosphorylated on serine residues, this modification is not required for DNA binding, but may affect its transactivation potential. Thus, Oct3/4 is a transcription factor that plays an important role in determining early steps of embryogenesis and differentiation. |
| Cat.No. | Product Name | Clone No. |
|---|
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
