Purified Anti-Mouse CD4 Antibody[RM4-4] (AN004170P)
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For research use only.
Conjugation: Unconjugated
APC Elab Fluor®488 Elab Fluor®647 Elab Fluor®Violet 450 FITC PE PE/Cyanine 5 PE/Elab Fluor®594 PerCP/Cyanine 5.5 Unconjugated Show More
| Alternate Names | L3T, CD4mut, p55, T-cell surface antigen T4/Leu, T-cell surface glycoprotein CD, L3T4, Ly-4, T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, Cd4, L3T4 |
| Clone | |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes cell |
| Dilution | FCM ≤ 0.2 µg per million cells in 100 µL volume |
| Background | CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. |
| Immunogen | Recombinant Mouse CD4 protein |
| Abbre | CD4 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2b, κ |
| Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2], Functional Grade |
| Applications |
FCM
|
| Cellular Localization |
Cell membrane
|
| Tissue Specificity |
Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes.
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | Unconjugated |
| Storage Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Cat.No. | Product Name | Clone No. |
|---|---|---|
| E-AB-F1097A | Purified Anti-Mouse CD4 Antibody[GK1.5] | GK1.5 |
| E-AB-F13530P | Purified Anti-Mouse CD4 Antibody[RM4-5] | RM4-5 |
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