QuicKey Pro Canine E3(Estriol) ELISA Kit (E-OSEL-C0003)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 1.17 pg/mL |
| Detection Range | 3.13-200 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 1 h 30 min |
| Reactivity | Canine |
| Specificity | This kit recognizes Canine Pg in samples.No significant cross-reactivity or interference between Canine Pg and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | serum, plasma |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine Pg are added to the micro ELISA plate wells. Canine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Canine Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
It is one of the estrogens secreted by the ovaries and is distinct from estrone (E1) and estradiol (E2). Estriol can regulate fetal intrauterine development and affect the sensitivity of pregnant uterus to oxytocin.
In addition, estriol is also used for non-hormonal treatments, such as non-hormonal vaginal renewal.
In clinical practice, estriol is often used as an adjuvant evaluation of abnormal pregnancy and adjuvant treatment of Down syndrome. However, it is important to note that although estriol can stimulate breast cell proliferation, its effect may not be as strong as estradiol, so it should only be used as a short-term therapy.
In summary, estriol is an important estrogen, mainly produced during pregnancy and menopause, and may be beneficial for some specific conditions of hormone deficiency or related diseases
| Research Area | Cell Biology , Signal Transduction , Developmental Biology |
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