QuicKey Pro Human LEP (Leptin) ELISA Kit (E-OSEL-H0022)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LEP are added to the micro ELISA plate wells. Human LEP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human LEP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LEP. The concentration of Human LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

LEP is key player in the regulation of energy balance and body weight control. Once released into the circulation,has central and peripheral effects by binding LEPR,found in many tissues,which results in the activation of several major signaling pathways.In the hypothalamus,acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides,also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery,increases basal metabolism,influences reproductive function,regulates pancreatic beta-cell function and insulin secretion,is pro-angiogenic for endothelial cell and affects innate and adaptive immunity.In the arcuate nucleus of the hypothalamus,activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides.In addition to its known satiety inducing effect,has a modulatory role in nutrient absorption. In the intestine,reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38,PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption.Acts as a growth factor on certain tissues,through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1,via JAK2-STAT3 pathway,or VEGFA,via MAPK1/3 and PI3K-AKT1 pathways.May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression.Pro-angiogenic,has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs).In innate immunity,modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells.