QuicKey Pro Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit (E-OSEL-H0086)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1.Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human MCP-1 are added to the micro ELISA plate wells. Human MCP-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human MCP-1. Excess conjugate and unbound sample or standard are washed from the plate.Upon reaction with a chromogenic substrate, the HRP enzyme catalyzes the development of a colored product. The resulting signal intensity, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Human MCP-1 in the sample.Quantitative determination is achieved by comparison with a standard curve generated from known concentrations.

Human MCP-1 also known as monocyte chemoattractant protein-1 or CCL2 is a small cytokine that belongs to the CC chemokine family. This protein with a molecular mass of approximately 13 kDa is a monomeric polypeptide. It is produced in endothelial cells, smooth muscle cells, and monocytes in response to inflammatory stimuli. MCP-1 functions as a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils to sites of inflammation. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.