QuicKey Pro Human VCAM-1/CD106 (Vascular Cell Adhesion Molecule 1) ELISA Kit (E-OSEL-H0038)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAM-1/CD106. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human VCAM-1/CD106 are added to the micro ELISA plate wells. Human VCAM-1/CD106 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human VCAM-1/CD106.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAM-1/CD106. The concentration of Human VCAM-1/CD106 in the samples is then determined by comparing the OD of the samples to the standard curve.

VCAM1 gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.