QuicKey Pro Human VWF (Von Willebrand Factor) ELISA Kit (E-OSEL-H0043)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VWF. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human VWF are added to the micro ELISA plate wells. Human VWF in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human VWF.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VWF. The concentration of Human VWF in the samples is then determined by comparing the OD of the samples to the standard curve.

The glycoprotein encoded by VWF gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.