Rat ABCA1 (ATP Binding Cassette Transporter A1) CLIA Kit (E-CL-R0062)

Name: Rat ABCA1 (ATP Binding Cassette Transporter A1) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0062
Price: 180.0 USD
Availability: InStock

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Rat ABCA1 (ATP Binding Cassette Transporter A1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat ABCA 1 in samples.No significant cross-reactivity or interference between Rat ABCA 1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	51451.000	61553.000	56502.000	56462
1000	23553.000	24533.000	24043.000	24003
500	12304.000	11820.000	12062.000	12022
250	5321.000	5385.000	5353.000	5313
125	2802.000	2556.000	2679.000	2639
62.5	1470.000	1328.000	1399.000	1359
31.25	703.000	757.000	730.000	690
0	39.000	41.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	91.890	313.020	845.830	98.380	298.670	868.720
Standard deviation	11.580	27.640	88.900	11.260	35.660	99.210
CV（%）	12.600	8.830	10.510	11.450	11.940	11.420

Recovery

The recovery of Rat ABCA1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-104	99
EDTA plasma （n=8）	91-106	97
Cell culture media （n=8）	102-118	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat ABCA1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-118	88-99	88-103
1:2	Average	108	94	96
1:4	Range	86-99	93-111	85-100
1:4	Average	92	101	92
1:8	Range	93-103	86-99	102-116
1:8	Average	98	93	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ABCA 1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ABCA 1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ABCA 1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat ABCA 1. You can calculate the concentration of Rat ABCA 1 in the samples by comparing the RLU value of the samples to the standard curve.

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