Rat AGER(Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit (E-EL-R0643)

Name: Rat AGER(Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit
Brand: Elabscience
SKU: E-EL-R0643
Price: 150.0 USD
Availability: InStock

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Rat AGER(Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat AGER in samples. No significant cross-reactivity or interference between Rat AGER and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.407	2.419	2.413	2.345
1000	1.692	1.698	1.695	1.627
500	0.979	0.963	0.971	0.903
250	0.487	0.489	0.488	0.420
125	0.265	0.255	0.260	0.192
62.5	0.180	0.160	0.170	0.102
31.25	0.117	0.123	0.120	0.052
0	0.063	0.073	0.068	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	106.400	227.000	906.100	104.000	220.000	849.000
Standard deviation	7.200	13.600	35.300	7.300	10.300	32.300
CV（%）	6.770	5.990	3.900	7.020	4.680	3.800

Recovery

The recovery of Rat AGER was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	86-96	91
Serum （n=8）	84-96	91
EDTA plasma （n=8）	86-101	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat AGER and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-102	95-111	86-99
1:2	Average	95	101	93
1:4	Range	97-113	84-98	99-113
1:4	Average	105	91	106
1:8	Range	90-106	86-96	98-112
1:8	Average	97	91	105

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	88.81-103.03
Sample 2（n=16）	92.32-107.00

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AGER. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AGER. You can calculate the concentration of Rat AGER in the samples by comparing the OD of the samples to the standard curve.

Background

Receptor for advanced glycation end products or AGER is a multi-ligand receptor that belongs to the immunoglobulin superfamily of cell surface molecules. AGER can be found in adult lung and skin tissue but low levels are found in other tissue types such as endothelia, cardiomyocytes, neutrophils, macrophages, lymphocytes, dendritic cells, and the central nervous system. AGER activation induces a positive feedback response to AGER leading to increase in expression of AGER regardless of expression levels. AGER has been found to be involved in pathogenesis and complications that are age related such as diabetes and Alzheimer’s, as well as being involved in cellular processes such as inflammation, apoptosis, autophagy, and proliferation.