Rat AGER(Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit (E-EL-R0643)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AGER. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AGER. You can calculate the concentration of Rat AGER in the samples by comparing the OD of the samples to the standard curve.

Receptor for advanced glycation end products or AGER is a multi-ligand receptor that belongs to the immunoglobulin superfamily of cell surface molecules. AGER can be found in adult lung and skin tissue but low levels are found in other tissue types such as endothelia, cardiomyocytes, neutrophils, macrophages, lymphocytes, dendritic cells, and the central nervous system. AGER activation induces a positive feedback response to AGER leading to increase in expression of AGER regardless of expression levels. AGER has been found to be involved in pathogenesis and complications that are age related such as diabetes and Alzheimer’s, as well as being involved in cellular processes such as inflammation, apoptosis, autophagy, and proliferation.