Rat AgRP (Agouti Related Protein) CLIA Kit (E-CL-R0744)

Name: Rat AgRP (Agouti Related Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0744
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat AgRP (Agouti Related Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat AgRP in samples.No significant cross-reactivity or interference between Rat AgRP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	55313.000	57593.000	56453.000	56414
250	20776.000	23952.000	22364.000	22325
125	10348.000	9522.000	9935.000	9896
62.5	4525.000	5117.000	4821.000	4782
31.25	2619.000	2379.000	2499.000	2460
15.63	1336.000	1272.000	1304.000	1265
7.81	743.000	793.000	768.000	729
0	37.000	41.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	26.240	52.580	179.680	27.100	55.070	165.200
Standard deviation	2.750	4.430	18.740	2.550	4.200	13.250
CV（%）	10.480	8.430	10.430	9.410	7.630	8.020

Recovery

The recovery of Rat AgRP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-100	92
EDTA plasma （n=8）	91-107	98
Cell culture media （n=8）	100-117	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat AgRP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-110	94-106	102-114
1:2	Average	103	100	108
1:4	Range	90-105	99-115	89-104
1:4	Average	96	108	96
1:8	Range	95-106	96-108	96-110
1:8	Average	101	102	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AgRP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat AgRP. You can calculate the concentration of Rat AgRP in the samples by comparing the RLU value of the samples to the standard curve.

Background