Rat ApoA1 (Apolipoprotein A1) CLIA Kit (E-CL-R0710)

Name: Rat ApoA1 (Apolipoprotein A1) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0710
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat ApoA1 (Apolipoprotein A1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	3.75 ng/mL
Detection Range	6.25-400 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat ApoA1 in samples.No significant cross-reactivity or interference between Rat ApoA1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
400	47942.000	55548.000	51745.000	51714
200	19104.000	20692.000	19898.000	19867
100	9328.000	8046.000	8687.000	8656
50	3942.000	4500.000	4221.000	4190
25	2238.000	2172.000	2205.000	2174
12.5	1181.000	1179.000	1180.000	1149
6.25	693.000	769.000	731.000	700
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	21.680	53.680	181.770	22.000	57.040	196.280
Standard deviation	1.780	5.000	12.360	2.570	5.400	16.980
CV（%）	8.210	9.310	6.800	11.680	9.470	8.650

Recovery

The recovery of Rat ApoA1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-98	91
EDTA plasma （n=8）	94-107	99
Cell culture media （n=8）	95-108	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat ApoA1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-112	91-106	99-115
1:2	Average	103	97	105
1:4	Range	85-99	99-115	96-111
1:4	Average	91	107	102
1:8	Range	96-108	98-113	96-110
1:8	Average	102	106	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoA1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat ApoA1. You can calculate the concentration of Rat ApoA1 in the samples by comparing the RLU value of the samples to the standard curve.

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