Rat ApoB (Apolipoprotein B) CLIA Kit (E-CL-R0717)

Name: Rat ApoB (Apolipoprotein B) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0717
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat ApoB (Apolipoprotein B) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 ng/mL
Detection Range	12.5-800 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat ApoB in samples.No significant cross-reactivity or interference between Rat ApoB and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
800	51996.000	58814.000	55405.000	55368
400	21243.000	23349.000	22296.000	22259
200	10580.000	9098.000	9839.000	9802
100	4636.000	5110.000	4873.000	4836
50	2409.000	2227.000	2318.000	2281
25	1191.000	1035.000	1113.000	1076
12.5	512.000	524.000	518.000	481
0	36.000	38.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	38.990	72.230	335.060	42.280	70.510	322.120
Standard deviation	3.320	5.420	26.370	3.800	5.380	33.470
CV（%）	8.520	7.500	7.870	8.990	7.630	10.390

Recovery

The recovery of Rat ApoB was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-111	104
EDTA plasma （n=8）	84-99	91
Cell culture media （n=8）	90-103	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat ApoB and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-117	87-98	89-100
1:2	Average	110	92	94
1:4	Range	93-108	100-114	89-105
1:4	Average	99	108	97
1:8	Range	88-105	92-105	88-104
1:8	Average	96	99	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoB. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat ApoB. You can calculate the concentration of Rat ApoB in the samples by comparing the RLU value of the samples to the standard curve.

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