Rat AR (Amphiregulin) CLIA Kit (E-CL-R0408)

Name: Rat AR (Amphiregulin) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0408
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat AR in samples.No significant cross-reactivity or interference between Rat AR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52037.000	54335.000	53186.000	53147
400	23333.000	23667.000	23500.000	23461
200	10524.000	9030.000	9777.000	9738
100	4746.000	5178.000	4962.000	4923
50	2552.000	2250.000	2401.000	2362
25	1156.000	1078.000	1117.000	1078
12.5	506.000	544.000	525.000	486
0	38.000	40.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	36.790	83.960	384.980	39.360	88.840	368.180
Standard deviation	4.610	9.130	28.600	3.480	10.260	23.270
CV（%）	12.530	10.870	7.430	8.840	11.550	6.320

Recovery

The recovery of Rat AR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-116	107
EDTA plasma （n=8）	92-104	98
Cell culture media （n=8）	94-112	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat AR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-115	91-103	88-102
1:2	Average	105	98	95
1:4	Range	102-115	102-119	103-116
1:4	Average	109	110	109
1:8	Range	95-107	96-110	96-109
1:8	Average	100	102	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat AR. You can calculate the concentration of Rat AR in the samples by comparing the RLU value of the samples to the standard curve.

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