Rat BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit (E-CL-R0082)

Name: Rat BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0082
Price: 180.0 USD
Availability: InStock

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Rat BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat BMP-4 in samples.No significant cross-reactivity or interference between Rat BMP-4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	51517.000	56319.000	53918.000	53886
250	21075.000	22525.000	21800.000	21768
125	10296.000	9502.000	9899.000	9867
62.5	4431.000	5157.000	4794.000	4762
31.25	2617.000	2279.000	2448.000	2416
15.63	1372.000	1304.000	1338.000	1306
7.81	728.000	812.000	770.000	738
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	27.060	68.940	230.380	28.780	64.060	220.640
Standard deviation	2.220	6.110	24.370	3.000	4.680	17.940
CV（%）	8.200	8.860	10.580	10.420	7.310	8.130

Recovery

The recovery of Rat BMP-4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-114	104
EDTA plasma （n=8）	89-103	95
Cell culture media （n=8）	87-101	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat BMP-4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	88-103	97-110
1:2	Average	101	95	104
1:4	Range	85-96	92-104	97-109
1:4	Average	91	99	103
1:8	Range	98-113	88-102	99-111
1:8	Average	106	96	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat BMP-4. You can calculate the concentration of Rat BMP-4 in the samples by comparing the RLU value of the samples to the standard curve.

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