Rat CA9 (Carbonic Anhydrase Ⅸ) CLIA Kit (E-CL-R0207)

Name: Rat CA9 (Carbonic Anhydrase Ⅸ) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0207
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat CA9 (Carbonic Anhydrase Ⅸ) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat CA9 in samples.No significant cross-reactivity or interference between Rat CA9 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	53061.000	54963.000	54012.000	53987
400	21311.000	24565.000	22938.000	22913
200	10851.000	9117.000	9984.000	9959
100	4605.000	5091.000	4848.000	4823
50	2376.000	2206.000	2291.000	2266
25	1160.000	1112.000	1136.000	1111
12.5	526.000	582.000	554.000	529
0	25.000	25.000	25.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	41.850	104.240	289.600	38.430	100.850	267.240
Standard deviation	5.230	11.660	22.390	3.730	9.320	25.710
CV（%）	12.500	11.190	7.730	9.710	9.240	9.620

Recovery

The recovery of Rat CA9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-102	93
EDTA plasma （n=8）	92-107	100
Cell culture media （n=8）	94-105	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CA9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-110	89-102	99-116
1:2	Average	103	96	106
1:4	Range	95-111	103-119	98-115
1:4	Average	102	109	105
1:8	Range	94-105	94-107	98-114
1:8	Average	99	99	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CA9. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CA9, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CA9. You can calculate the concentration of Rat CA9 in the samples by comparing the RLU value of the samples to the standard curve.