Rat CEACAM1(Carcinoembryonic Antigen Related Cell Adhesion Molecule 1) ELISA Kit (E-EL-R3082)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CEACAM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CEACAM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CEACAM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CEACAM1. You can calculate the concentration of CEACAM1 in the samples by comparing the OD of the samples to the standard curve.

Carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1), also termed CD66a, is a glycoprotein belonging to the immunoglobulin superfamily and CEA gene family in rats. Initially identified as hepatic biliary glycoprotein, it is widely expressed in leukocytes, epithelia and endothelia, as well as multiple malignant tissues including gastric, colorectal, pancreatic, non-small cell lung cancers and melanoma.Structurally, rat CEACAM1 consists of an N-terminal variable domain, six C2-like Ig domains and a GPI linker, mediating cell adhesion via homophilic binding and heterophilic interactions with CEACAM5. It participates in diverse biological processes, including tissue structural remodeling, angiogenesis, apoptosis, and regulation of innate and adaptive immunity.As a critical tumor biomarker for gastrointestinal and respiratory malignancies, CEACAM1 exerts dual regulatory effects on tumor initiation, progression and metastasis, and serves as a vital tumor cell growth inhibitor with important clinical research value in rat tumor models.