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Rat COL1 (Collagen Type Ⅰ) CLIA Kit (E-CL-R0159)

All Size Price Qty
96T $ 682.00
48T $ 546.00
24T $ 180.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.19 ng/mL
Detection Range 0.31-20 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Rat
Specificity This kit recognizes Rat COL1 in samples.No significant cross-reactivity or interference between Rat COL1 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Chemiluminescence
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 6 months
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat COL1. You can calculate the concentration of Rat COL1 in the samples by comparing the RLU value of the samples to the standard curve.
Uniport ID P02454
Research Area Signal Transduction , Stem Cells
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