Rat COL1 (Collagen Type Ⅰ) CLIA Kit (E-CL-R0159)

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Size	Price	Qty
96T	$ 682.00
48T	$ 546.00
24T	$ 180.00
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reacitivity	Rat
Specificity	This kit recognizes Rat COL1 in samples.No significant cross-reactivity or interference between Rat COL1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat COL1. You can calculate the concentration of Rat COL1 in the samples by comparing the RLU value of the samples to the standard curve.

Background

Uniport ID	P02454
Research Area	Signal Transduction , Stem Cells

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Rat COL1 (Collagen Type Ⅰ) CLIA Kit (E-CL-R0159)

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