Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit (E-CL-R0721)

Name: Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0721
Price: 180.0 USD
Availability: InStock

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Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat cTn-I/TNNI3 in samples.No significant cross-reactivity or interference between Rat cTn-I/TNNI3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	55837.000	58455.000	57146.000	57113
400	20055.000	23451.000	21753.000	21720
200	10533.000	9699.000	10116.000	10083
100	4415.000	5035.000	4725.000	4692
50	2510.000	2302.000	2406.000	2373
25	1148.000	1138.000	1143.000	1110
12.5	485.000	539.000	512.000	479
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	43.140	127.670	380.900	42.340	115.950	403.720
Standard deviation	4.310	14.450	34.930	5.400	10.410	45.380
CV（%）	9.990	11.320	9.170	12.750	8.980	11.240

Recovery

The recovery of Rat cTn-I was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-103	95
EDTA plasma （n=8）	95-109	103
Cell culture media （n=8）	89-102	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat cTn-I and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-115	85-96	87-102
1:2	Average	109	91	94
1:4	Range	98-112	93-105	98-114
1:4	Average	105	99	107
1:8	Range	87-98	102-117	87-102
1:8	Average	93	108	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat cTn-I/TNNI3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTn-I/TNNI3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTn-I/TNNI3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat cTn-I/TNNI3. You can calculate the concentration of Rat cTn-I/TNNI3 in the samples by comparing the RLU value of the samples to the standard curve.

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