Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) CLIA Kit (E-CL-R0747)

Name: Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0747
Price: 180.0 USD
Availability: InStock

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Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat CTXI in samples.No significant cross-reactivity or interference between Rat CTXI and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58312.000	63042.000	60677.000	60646
1000	24291.000	28137.000	26214.000	26183
500	11811.000	10939.000	11375.000	11344
250	5201.000	5545.000	5373.000	5342
125	2826.000	2508.000	2667.000	2636
62.5	1355.000	1333.000	1344.000	1313
31.25	672.000	678.000	675.000	644
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	106.060	300.110	823.040	101.780	300.410	853.990
Standard deviation	12.110	26.440	52.020	11.550	29.710	56.960
CV（%）	11.420	8.810	6.320	11.350	9.890	6.670

Recovery

The recovery of Rat CTXI was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-104	98
EDTA plasma （n=8）	96-111	104
Cell culture media （n=8）	92-106	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CTXI and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-96	103-119	85-96
1:2	Average	90	109	90
1:4	Range	86-100	88-98	84-97
1:4	Average	91	93	91
1:8	Range	95-108	103-120	95-111
1:8	Average	100	110	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXI. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXI, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CTXI. You can calculate the concentration of Rat CTXI in the samples by comparing the RLU value of the samples to the standard curve.

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