Rat DBP (Vitamin D Binding Protein) CLIA Kit (E-CL-R0692)

Name: Rat DBP (Vitamin D Binding Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0692
Price: 180.0 USD
Availability: InStock

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Rat DBP (Vitamin D Binding Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	1.88 ng/mL
Detection Range	3.13-200 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat DBP in samples.No significant cross-reactivity or interference between Rat DBP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
200	44873.000	46333.000	45603.000	45572
100	17890.000	21430.000	19660.000	19629
50	8742.000	8688.000	8715.000	8684
25	3863.000	4471.000	4167.000	4136
12.5	2242.000	2052.000	2147.000	2116
6.25	1190.000	1062.000	1126.000	1095
3.13	620.000	666.000	643.000	612
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	10.780	24.970	98.820	11.710	24.430	103.120
Standard deviation	1.120	2.840	9.630	1.270	2.740	11.310
CV（%）	10.390	11.370	9.740	10.850	11.220	10.970

Recovery

The recovery of Rat DBP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-104	97
EDTA plasma （n=8）	96-111	101
Cell culture media （n=8）	98-111	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat DBP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-116	103-121	92-107
1:2	Average	107	110	99
1:4	Range	99-116	101-115	100-117
1:4	Average	107	107	108
1:8	Range	86-98	99-112	91-104
1:8	Average	93	105	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DBP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DBP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat DBP. You can calculate the concentration of Rat DBP in the samples by comparing the RLU value of the samples to the standard curve.

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