Rat DRD3 (Dopamine Receptor D3) CLIA Kit (E-CL-R0221)

Name: Rat DRD3 (Dopamine Receptor D3) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0221
Price: 180.0 USD
Availability: InStock

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Rat DRD3 (Dopamine Receptor D3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat DRD3 in samples.No significant cross-reactivity or interference between Rat DRD3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	62251.000	63293.000	62772.000	62748
2000	27585.000	33333.000	30459.000	30435
1000	14886.000	13026.000	13956.000	13932
500	6735.000	6739.000	6737.000	6713
250	3536.000	3492.000	3514.000	3490
125	1637.000	1535.000	1586.000	1562
62.5	727.000	799.000	763.000	739
0	24.000	24.000	24.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	219.850	510.660	1716.980	216.220	557.030	1623.010
Standard deviation	23.280	56.270	174.790	24.710	58.880	148.990
CV（%）	10.590	11.020	10.180	11.430	10.570	9.180

Recovery

The recovery of Rat DRD3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-112	103
EDTA plasma （n=8）	88-101	95
Cell culture media （n=8）	99-115	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat DRD3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-115	86-100	99-113
1:2	Average	105	92	107
1:4	Range	88-104	96-110	89-102
1:4	Average	96	102	96
1:8	Range	88-100	90-105	90-102
1:8	Average	93	96	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DRD3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat DRD3. You can calculate the concentration of Rat DRD3 in the samples by comparing the RLU value of the samples to the standard curve.

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