Rat F2 (Coagulation Factor Ⅱ) CLIA Kit (E-CL-R0151)

Name: Rat F2 (Coagulation Factor Ⅱ) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0151
Price: 180.0 USD
Availability: InStock

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Rat F2 (Coagulation Factor Ⅱ) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat F2 in samples.No significant cross-reactivity or interference between Rat F2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55166.000	56646.000	55906.000	55864
1000	24911.000	25027.000	24969.000	24927
500	11474.000	11454.000	11464.000	11422
250	5514.000	5812.000	5663.000	5621
125	2929.000	2517.000	2723.000	2681
62.5	1447.000	1337.000	1392.000	1350
31.25	689.000	725.000	707.000	665
0	40.000	44.000	42.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	103.170	317.780	775.850	94.180	299.370	812.570
Standard deviation	9.110	28.820	80.920	9.950	21.170	68.420
CV（%）	8.830	9.070	10.430	10.560	7.070	8.420

Recovery

The recovery of Rat F2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-110	100
EDTA plasma （n=8）	93-108	99
Cell culture media （n=8）	97-112	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat F2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-120	90-105	101-117
1:2	Average	110	96	107
1:4	Range	97-110	93-105	91-105
1:4	Average	104	98	97
1:8	Range	100-117	89-103	95-108
1:8	Average	108	97	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat F2. You can calculate the concentration of Rat F2 in the samples by comparing the RLU value of the samples to the standard curve.

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