Rat GDH/GLDH(Glutamate Dehydrogenase) CLIA Kit (E-CL-R0292)
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For research use only.
Product Summary
| Sensitivity | 75 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 4 h 30 min |
| Reactivity | Rat |
| Specificity | This kit recognizes Rat GDH/GLDH in samples.No significant cross-reactivity or interference between Rat GDH/GLDH and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Chemiluminescence |
| Assay Type | Sandwich-CLIA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃/-20℃ |
| Expiration Date | 6 months |
Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDH/GLDH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDH/GLDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDH/GLDH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GDH/GLDH. You can calculate the concentration of Rat GDH/GLDH in the samples by comparing the RLU value of the samples to the standard curve.
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