Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit (E-CL-R0740)

Name: Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0740
Price: 180.0 USD
Availability: InStock

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Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat GFAP in samples.No significant cross-reactivity or interference between Rat GFAP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	64014.000	64706.000	64360.000	64324
2000	29178.000	29784.000	29481.000	29445
1000	14682.000	12772.000	13727.000	13691
500	6278.000	7450.000	6864.000	6828
250	3478.000	3212.000	3345.000	3309
125	1862.000	1602.000	1732.000	1696
62.5	793.000	811.000	802.000	766
0	36.000	36.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	198.820	517.360	1456.270	214.620	541.210	1491.350
Standard deviation	16.220	43.460	156.260	26.270	59.700	141.680
CV（%）	8.160	8.400	10.730	12.240	11.030	9.500

Recovery

The recovery of Rat GFAP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-107	97
EDTA plasma （n=8）	84-100	91
Cell culture media （n=8）	101-117	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat GFAP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	94-107	93-107
1:2	Average	96	100	100
1:4	Range	87-100	86-97	101-118
1:4	Average	94	92	108
1:8	Range	89-102	92-107	98-112
1:8	Average	96	98	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GFAP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GFAP. You can calculate the concentration of Rat GFAP in the samples by comparing the RLU value of the samples to the standard curve.

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