Rat GSTA1 (Glutathione S Transferase Alpha 1) CLIA Kit (E-CL-R0294)

Name: Rat GSTA1 (Glutathione S Transferase Alpha 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0294
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat GSTA1 (Glutathione S Transferase Alpha 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat GSTA1 in samples.No significant cross-reactivity or interference between Rat GSTA1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	58391.000	65385.000	61888.000	61852
2000	29267.000	29761.000	29514.000	29478
1000	13767.000	13413.000	13590.000	13554
500	6703.000	6949.000	6826.000	6790
250	3648.000	3230.000	3439.000	3403
125	1671.000	1643.000	1657.000	1621
62.5	790.000	808.000	799.000	763
0	34.000	38.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	193.380	567.340	1987.610	174.420	618.890	2097.280
Standard deviation	16.070	66.610	183.060	22.590	45.490	209.520
CV（%）	8.310	11.740	9.210	12.950	7.350	9.990

Recovery

The recovery of Rat GSTA1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-98	91
EDTA plasma （n=8）	87-102	94
Cell culture media （n=8）	94-109	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat GSTA1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-106	104-116	99-111
1:2	Average	97	110	105
1:4	Range	88-100	102-118	103-115
1:4	Average	94	108	109
1:8	Range	86-99	92-108	103-117
1:8	Average	92	98	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GSTA1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GSTA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GSTA1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GSTA1. You can calculate the concentration of Rat GSTA1 in the samples by comparing the RLU value of the samples to the standard curve.