Rat HPX (Hemopexin) CLIA Kit (E-CL-R0335)

Name: Rat HPX (Hemopexin) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0335
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat HPX in samples.No significant cross-reactivity or interference between Rat HPX and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	58177.000	58823.000	58500.000	58464
500	22756.000	24520.000	23638.000	23602
250	10815.000	10097.000	10456.000	10420
125	4766.000	5280.000	5023.000	4987
62.5	2683.000	2645.000	2664.000	2628
31.25	1409.000	1263.000	1336.000	1300
15.63	737.000	799.000	768.000	732
0	36.000	36.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	54.630	132.490	470.020	51.680	140.910	486.680
Standard deviation	5.220	9.620	45.690	5.500	16.180	54.360
CV（%）	9.560	7.260	9.720	10.640	11.480	11.170

Recovery

The recovery of Rat HPX was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	101-115	106
EDTA plasma （n=8）	93-108	100
Cell culture media （n=8）	93-106	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat HPX and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-96	84-98	90-105
1:2	Average	91	91	97
1:4	Range	86-99	100-112	85-97
1:4	Average	91	106	92
1:8	Range	96-111	96-112	92-109
1:8	Average	102	103	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HPX. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HPX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HPX, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat HPX. You can calculate the concentration of Rat HPX in the samples by comparing the RLU value of the samples to the standard curve.

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