Rat HSP-60 (Heat Shock Protein 60) CLIA Kit (E-CL-R0325)

Name: Rat HSP-60 (Heat Shock Protein 60) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0325
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat HSP-60 (Heat Shock Protein 60) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat Hsp-60 in samples.No significant cross-reactivity or interference between Rat Hsp-60 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	62911.000	64093.000	63502.000	63468
2000	28641.000	32367.000	30504.000	30470
1000	15597.000	13777.000	14687.000	14653
500	6664.000	6872.000	6768.000	6734
250	3730.000	3250.000	3490.000	3456
125	1613.000	1599.000	1606.000	1572
62.5	766.000	850.000	808.000	774
0	32.000	36.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	201.950	518.970	1385.440	184.650	563.890	1265.860
Standard deviation	17.490	46.710	151.150	15.220	41.050	136.330
CV（%）	8.660	9.000	10.910	8.240	7.280	10.770

Recovery

The recovery of Rat Hsp-60 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-99	92
EDTA plasma （n=8）	99-112	105
Cell culture media （n=8）	97-112	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat Hsp-60 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-108	102-118	96-108
1:2	Average	99	109	103
1:4	Range	93-110	88-100	93-109
1:4	Average	101	94	101
1:8	Range	88-99	89-103	94-109
1:8	Average	94	95	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Hsp-60. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Hsp-60 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Hsp-60, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat Hsp-60. You can calculate the concentration of Rat Hsp-60 in the samples by comparing the RLU value of the samples to the standard curve.

Background