Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit (E-CL-R0431)

Name: Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0431
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat MIP-1α in samples.No significant cross-reactivity or interference between Rat MIP-1α and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	55350.000	57752.000	56551.000	56520
250	19932.000	24224.000	22078.000	22047
125	11153.000	9321.000	10237.000	10206
62.5	4340.000	4816.000	4578.000	4547
31.25	2478.000	2140.000	2309.000	2278
15.63	1293.000	1283.000	1288.000	1257
7.81	739.000	801.000	770.000	739
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	27.680	69.550	169.720	27.720	63.980	167.980
Standard deviation	2.670	6.950	15.380	3.300	6.820	12.800
CV（%）	9.650	9.990	9.060	11.900	10.660	7.620

Recovery

The recovery of Rat MIP-1α was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-107	99
EDTA plasma （n=8）	88-101	95
Cell culture media （n=8）	93-107	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat MIP-1α and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-104	91-108	84-99
1:2	Average	96	98	91
1:4	Range	83-97	99-113	95-109
1:4	Average	90	107	101
1:8	Range	103-119	89-103	94-109
1:8	Average	109	95	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MIP-1α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIP-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat MIP-1α. You can calculate the concentration of Rat MIP-1α in the samples by comparing the RLU value of the samples to the standard curve.

Background