Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit (E-CL-R0522)

Name: Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0522
Price: 180.0 USD
Availability: InStock

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Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat PAI-1 in samples.No significant cross-reactivity or interference between Rat PAI-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	60020.000	63556.000	61788.000	61755
2000	26619.000	31741.000	29180.000	29147
1000	15266.000	14068.000	14667.000	14634
500	6720.000	7608.000	7164.000	7131
250	3488.000	3354.000	3421.000	3388
125	1620.000	1592.000	1606.000	1573
62.5	793.000	825.000	809.000	776
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	204.050	466.610	1710.000	195.090	481.940	1692.650
Standard deviation	25.000	47.220	141.250	22.630	43.570	141.670
CV（%）	12.250	10.120	8.260	11.600	9.040	8.370

Recovery

The recovery of Rat PAI-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-111	102
EDTA plasma （n=8）	86-98	92
Cell culture media （n=8）	95-110	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat PAI-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-106	97-111	95-109
1:2	Average	99	105	101
1:4	Range	99-116	92-105	85-99
1:4	Average	106	98	90
1:8	Range	101-118	86-100	97-114
1:8	Average	109	93	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PAI-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PAI-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PAI-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PAI-1. You can calculate the concentration of Rat PAI-1 in the samples by comparing the RLU value of the samples to the standard curve.

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