Rat PGR (Progesterone Receptor) CLIA Kit (E-CL-R0549)

Name: Rat PGR (Progesterone Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0549
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat PGR (Progesterone Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat PGR in samples.No significant cross-reactivity or interference between Rat PGR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	57528.000	62200.000	59864.000	59834
2000	27854.000	28822.000	28338.000	28308
1000	13900.000	13372.000	13636.000	13606
500	6690.000	7424.000	7057.000	7027
250	3584.000	3456.000	3520.000	3490
125	1674.000	1642.000	1658.000	1628
62.5	745.000	807.000	776.000	746
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	204.480	585.450	1667.080	216.070	603.880	1555.980
Standard deviation	20.900	50.290	127.700	27.380	61.960	150.770
CV（%）	10.220	8.590	7.660	12.670	10.260	9.690

Recovery

The recovery of Rat PGR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-109	102
EDTA plasma （n=8）	99-111	105
Cell culture media （n=8）	93-107	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat PGR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-99	85-101	103-120
1:2	Average	91	92	110
1:4	Range	95-106	96-112	87-99
1:4	Average	100	102	94
1:8	Range	99-115	95-112	96-108
1:8	Average	106	103	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PGR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PGR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PGR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PGR. You can calculate the concentration of Rat PGR in the samples by comparing the RLU value of the samples to the standard curve.

Background