Rat PRL (Prolactin) CLIA Kit (E-CL-R0041)

Name: Rat PRL (Prolactin) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0041
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat PRL in samples.No significant cross-reactivity or interference between Rat PRL and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	30854.000	36266.000	33560.000	33527
5	15574.000	17466.000	16520.000	16487
2.5	8238.000	7594.000	7916.000	7883
1.25	3648.000	4268.000	3958.000	3925
0.63	1997.000	1813.000	1905.000	1872
0.31	894.000	798.000	846.000	813
0.16	361.000	389.000	375.000	342
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.490	1.120	4.910	0.490	1.200	5.290
Standard deviation	0.050	0.090	0.450	0.050	0.140	0.500
CV（%）	10.200	8.040	9.160	10.200	11.670	9.450

Recovery

The recovery of Rat PRL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-104	99
EDTA plasma （n=8）	99-112	105
Cell culture media （n=8）	99-114	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat PRL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-118	103-118	91-104
1:2	Average	110	110	96
1:4	Range	93-108	96-108	97-114
1:4	Average	101	102	104
1:8	Range	101-117	97-111	101-120
1:8	Average	109	104	110

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PRL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PRL. You can calculate the concentration of Rat PRL in the samples by comparing the RLU value of the samples to the standard curve.

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