Rat RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit (E-CL-R0587)

Name: Rat RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0587
Price: 180.0 USD
Availability: InStock

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Rat RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat RBP4 in samples.No significant cross-reactivity or interference between Rat RBP4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	36489.000	36803.000	36646.000	36613
10	11913.000	13029.000	12471.000	12438
5	5820.000	5670.000	5745.000	5712
2.5	2551.000	2785.000	2668.000	2635
1.25	1589.000	1433.000	1511.000	1478
0.63	1020.000	878.000	949.000	916
0.31	624.000	664.000	644.000	611
0	31.000	35.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.920	2.690	8.210	0.930	2.650	8.400
Standard deviation	0.090	0.200	0.820	0.080	0.260	0.880
CV（%）	9.780	7.430	9.990	8.600	9.810	10.480

Recovery

The recovery of Rat RBP4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-102	97
EDTA plasma （n=8）	101-116	106
Cell culture media （n=8）	86-100	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat RBP4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-112	85-98	90-103
1:2	Average	103	90	95
1:4	Range	88-101	99-110	89-103
1:4	Average	94	104	96
1:8	Range	100-116	101-113	87-99
1:8	Average	107	108	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RBP4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat RBP4. You can calculate the concentration of Rat RBP4 in the samples by comparing the RLU value of the samples to the standard curve.

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