Rat SEPP1 (Selenoprotein P) CLIA Kit (E-CL-R0599)

Name: Rat SEPP1 (Selenoprotein P) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0599
Price: 180.0 USD
Availability: InStock

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Rat SEPP1 (Selenoprotein P) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat SEPP1 in samples.No significant cross-reactivity or interference between Rat SEPP1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54703.000	58357.000	56530.000	56504
1000	24583.000	26395.000	25489.000	25463
500	11415.000	11151.000	11283.000	11257
250	5036.000	5694.000	5365.000	5339
125	2700.000	2570.000	2635.000	2609
62.5	1390.000	1304.000	1347.000	1321
31.25	654.000	712.000	683.000	657
0	26.000	26.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	95.940	243.480	697.140	92.310	219.580	762.370
Standard deviation	12.450	18.770	76.340	8.780	15.660	84.470
CV（%）	12.980	7.710	10.950	9.510	7.130	11.080

Recovery

The recovery of Rat SEPP1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-98	91
EDTA plasma （n=8）	84-97	90
Cell culture media （n=8）	87-99	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat SEPP1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-99	97-109	99-115
1:2	Average	93	102	105
1:4	Range	100-112	99-113	89-102
1:4	Average	106	105	96
1:8	Range	92-105	95-111	92-105
1:8	Average	100	103	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SEPP1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat SEPP1. You can calculate the concentration of Rat SEPP1 in the samples by comparing the RLU value of the samples to the standard curve.

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