Rat SHH (Sonic Hedgehog Protein) CLIA Kit (E-CL-R0331)

Name: Rat SHH (Sonic Hedgehog Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0331
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat SHH (Sonic Hedgehog Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat SHH in samples.No significant cross-reactivity or interference between Rat SHH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	52934.000	61938.000	57436.000	57413
1000	24053.000	25699.000	24876.000	24853
500	12746.000	11454.000	12100.000	12077
250	5015.000	5661.000	5338.000	5315
125	3057.000	2653.000	2855.000	2832
62.5	1482.000	1280.000	1381.000	1358
31.25	704.000	722.000	713.000	690
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	98.660	175.670	970.200	92.270	188.990	893.310
Standard deviation	9.360	19.340	66.360	10.850	18.160	78.520
CV（%）	9.490	11.010	6.840	11.760	9.610	8.790

Recovery

The recovery of Rat SHH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-103	94
EDTA plasma （n=8）	100-116	107
Cell culture media （n=8）	90-100	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat SHH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-111	100-116	86-101
1:2	Average	103	108	93
1:4	Range	94-105	85-98	90-103
1:4	Average	99	91	95
1:8	Range	97-115	89-99	96-110
1:8	Average	105	94	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SHH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SHH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat SHH. You can calculate the concentration of Rat SHH in the samples by comparing the RLU value of the samples to the standard curve.

Background