Rat SYP (Synaptophysin) CLIA Kit (E-CL-R0622)

Name: Rat SYP (Synaptophysin) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0622
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	75 pg/mL
Detection Range	125-8000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat SYP in samples.No significant cross-reactivity or interference between Rat SYP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
8000	74574.000	77342.000	75958.000	75925
4000	36825.000	37633.000	37229.000	37196
2000	19283.000	18499.000	18891.000	18858
1000	8288.000	9664.000	8976.000	8943
500	4276.000	4120.000	4198.000	4165
250	2030.000	1858.000	1944.000	1911
125	760.000	870.000	815.000	782
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	422.690	1221.320	2994.170	410.130	1268.110	2816.020
Standard deviation	54.060	111.510	277.260	48.270	116.540	202.470
CV（%）	12.790	9.130	9.260	11.770	9.190	7.190

Recovery

The recovery of Rat SYP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-103	94
EDTA plasma （n=8）	94-108	101
Cell culture media （n=8）	93-108	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat SYP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-109	100-115	93-104
1:2	Average	99	106	98
1:4	Range	94-105	91-105	96-111
1:4	Average	100	96	104
1:8	Range	85-96	96-110	88-99
1:8	Average	91	103	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SYP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SYP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SYP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat SYP. You can calculate the concentration of Rat SYP in the samples by comparing the RLU value of the samples to the standard curve.

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