Rat TP53 (Tumor Protein 53) CLIA Kit (E-CL-R0677)

Name: Rat TP53 (Tumor Protein 53) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0677
Price: 180.0 USD
Availability: InStock

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Rat TP53 (Tumor Protein 53) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat TP53 in samples.No significant cross-reactivity or interference between Rat TP53 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	51653.000	62055.000	56854.000	56819
500	21087.000	25731.000	23409.000	23374
250	11245.000	10133.000	10689.000	10654
125	5225.000	5481.000	5353.000	5318
62.5	2576.000	2448.000	2512.000	2477
31.25	1312.000	1286.000	1299.000	1264
15.63	756.000	772.000	764.000	729
0	34.000	36.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	54.570	129.740	409.470	56.200	135.660	428.000
Standard deviation	5.860	12.200	44.710	6.370	14.570	37.580
CV（%）	10.740	9.400	10.920	11.330	10.740	8.780

Recovery

The recovery of Rat TP53 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-101	92
EDTA plasma （n=8）	91-102	97
Cell culture media （n=8）	94-111	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat TP53 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-107	95-107	88-103
1:2	Average	100	101	95
1:4	Range	100-114	103-117	98-112
1:4	Average	106	108	105
1:8	Range	87-101	102-117	98-114
1:8	Average	93	109	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TP53. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat TP53. You can calculate the concentration of Rat TP53 in the samples by comparing the RLU value of the samples to the standard curve.

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