Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method) (E-BC-K225-M)
For research use only.
| Detection Principle | Fe3+ -TPTZ can be reduced by antioxidants and produce blue Fe2+ -TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm. |
| Synonyms | T-AOC |
| Sample Type | Serum,Plasma,Saliva,Urine,Tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-600 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.049 mmol/L |
| Detection Range | 0.049-2.5 mmol/L |
| Precision | inter-assay CV: 8.1% | intra-assay CV: 3.9% |
| Sample Volume | 5 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human saliva | 1 |
| Human urine | 1 |
| Cellular supernatant | 1 |
| HepG2 cells homogenization | 1 |
| 5% Mouse liver tissue homogenization | 1 |
| 10% Epipremnum aureum tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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