Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method) (E-BC-K261-M)
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For research use only.
| Detection Principle | Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of trinder’s reagent, hydrogen peroxide is catalyzed by peroxidase to produce quinones which is proportional to the content of TG. |
| Synonyms | TG |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (510 nm) |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Isopropanol (AR), Anhydrous ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.14 mmol/L |
| Detection Range | 0.14-10 mmol/L |
| Precision | inter-assay CV: 9.2% | intra-assay CV: 4.1% |
| Sample Volume | 2.5 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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