Tryptophan (Trp) Fluorometric Assay Kit (E-BC-F091)
For research use only.
| Detection Principle | Tryptophan (Trp) is an essential amino acid involved in a variety of biosynthetic processes. It is not only involved in the synthesis of a variety of proteins, but also the precursor of many bioactive substances. The enzyme catalyzes Trp in a series of reactions to produce hydrogen peroxide, which forms a red complex with the fluorescent probe. The fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm can be measured to calculate the content of Trp in the sample. |
| Synonyms | Trp |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.33 μmol/L |
| Detection Range | 0.33-200.00 μmol/L |
| Precision | inter-assay CV: 2.5-5.6% | intra-assay CV: 3.4-4.7% |
| Sample Volume | 40 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rabbit serum | 1 |
| Rat serum | 1 |
| Mouse plasma | 1 |
| Bovine serum | 1 |
| Human serum | 3-8 |
| Rat plasma | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 10% Rat liver tissue homogenization | 1 |
| 10% Rat heart tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 0.193 × 10^6 Hela cells | 1 |
| 0.7 × 10^6 Jurkat cells | 1 |
| 0.446 × 10^6 RAW cells | 1 |
| 0.708 × 10^6 Molt cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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