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Uncoated cAMP(Cyclic adenosine monophosphate) ELISA Kit (E-UNEL-0001)

Uncoated cAMP(Cyclic adenosine monophosphate) ELISA Kit - 1
  • Uncoated cAMP(Cyclic adenosine monophosphate) ELISA Kit - 1
  • Uncoated cAMP(Cyclic adenosine monophosphate) ELISA Kit - 2
  • Uncoated cAMP(Cyclic adenosine monophosphate) ELISA Kit - 3
All Size Price Qty
96T*5 $ 260.00
- +
96T*15 $ 550.00
- +
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For research use only.

Product Summary

The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range 1.56-100 ng/mL
Sample Volume 100 μL
Total Assay Time 24 h
Reactivity Universal
Specificity This kit recognizes cAMP in samples.No significant cross-reactivity or interference between cAMP and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T*5 / 96T*15
Storage -20℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Research Area Signal Transduction
Cat.No. Product Name Sizes
E-ELIR-K001 Ancillary Reagent Kit 96T*5
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