Uncoated Canine CRP (C-Reactive Protein) ELISA Kit (E-UNEL-C0001)

Name: Uncoated Canine CRP (C-Reactive Protein) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-C0001
Price: 399.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Uncoated Canine CRP (C-Reactive Protein) ELISA Kit - 1

Uncoated Canine CRP (C-Reactive Protein) ELISA Kit - 2

Uncoated Canine CRP (C-Reactive Protein) ELISA Kit - 3

Uncoated Canine CRP (C-Reactive Protein) ELISA Kit - 4

All	Size	Price	Qty
	96T*5	$ 399.00	- +
	96T*15	$ 958.00	- +

Ancillary Products Available: Ancillary Reagent Kit

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	1.56-100 ng/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Canine
Specificity	This kit recognizes Canine CRP in samples.No significant cross-reactivity or interference between Canine CRP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
100	2.226	2.270	2.248	2.162
50	1.402	1.416	1.409	1.323
25	0.995	0.955	0.975	0.889
12.5	0.511	0.501	0.506	0.42
6.25	0.308	0.296	0.302	0.216
3.13	0.178	0.186	0.182	0.096
1.56	0.143	0.133	0.138	0.052
0	0.087	0.085	0.086	-

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. Prior to the assay, the plate must be coated with antibody specific for Canine CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Canine CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Canine CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Canine CRP. You can calculate the concentration of Canine CRP in the samples by comparing the OD of the samples to the standard curve.

Background

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently; the level of this protein in plasma increases greatly during acute phase response to tissue injury; infection; or other inflammatory stimuli.

Research Area

CellBiology