Uncoated Canine IFN-γ (Interferon Gamma) ELISA Kit (E-UNEL-C0002)

Name: Uncoated Canine IFN-γ (Interferon Gamma) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-C0002
Price: 399.0 USD
Availability: InStock

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Uncoated Canine IFN-γ (Interferon Gamma) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 399.00	- +
	96T*15	$ 958.00	- +

Ancillary Products Available: Ancillary Reagent Kit

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Canine
Specificity	This kit recognizes Canine IFN-γ in samples.No significant cross-reactivity or interference between Canine IFN-γ and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.490	2.534	2.512	2.419
1000	1.739	1.709	1.724	1.631
500	0.951	0.993	0.972	0.879
250	0.551	0.545	0.548	0.455
125	0.321	0.335	0.328	0.235
62.5	0.203	0.189	0.196	0.103
31.25	0.150	0.142	0.146	0.053
0	0.090	0.096	0.093	-

Background

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial; antiviral; and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding; IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2; JAK1 and STAT1; leading to STAT1 activation; nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn; increases the quantity; quality; and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the express