Uncoated Canine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit (E-UNEL-C0004)

Name: Uncoated Canine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-C0004
Price: 399.0 USD
Availability: InStock

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Uncoated Canine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 399.00	- +
	96T*15	$ 958.00	- +

Ancillary Products Available: Ancillary Reagent Kit

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	6.25-400 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Canine
Specificity	This kit recognizes Canine TNF-α in samples.No significant cross-reactivity or interference between Canine TNF-α and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
400	2.243	2.151	2.197	2.119
200	1.682	1.670	1.676	1.598
100	0.952	0.930	0.941	0.863
50	0.537	0.531	0.534	0.456
25	0.311	0.299	0.305	0.227
12.5	0.173	0.185	0.179	0.101
6.25	0.137	0.127	0.132	0.054
0	0.079	0.077	0.078	-

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to; and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation; differentiation; apoptosis; lipid metabolism; and coagulation. This cytokine has been implicated in a variety of diseases; including autoimmune diseases; insulin resistance; and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.