Uncoated Human CD200 ( Cluster of Differentiation 200 ) ELISA Kit (E-UNEL-H0380)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD200 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD200 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD200 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CD200 . You can calculate the concentration of CD200 in the samples by comparing the OD of the samples to the standard curve.

CD200,also known as OX2,is a member of the immunoglobulin superfamily (IgSF). It is a monomorphic cell surface glycoprotein that is expressed on thymocytes,neurons,endothelium,follicular dendritic cells in all lymphoid organs,a subset of CD34+ progenitor cells,and at low levels on some smooth muscle and B lymphocytes. It is not expressed on NK cells,monocytes,granulocytes,or platelets. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. The interaction between CD200 (OX2) and CD200 receptor (OX2R) system is of importance in the control of macrophage and granulocyte activation,which may contribute to pathways that suppress and limit macrophage induced inflammatory damage in tissue.