Uncoated Human CTLA4 ( Cytotoxic T-Lymphocyte Associated Antigen 4 ) ELISA Kit (E-UNEL-H0381)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CTLA4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CTLA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CTLA4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CTLA4 . You can calculate the concentration of CTLA4 in the samples by comparing the OD of the samples to the standard curve.

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4),also known as CD152,is a protein that plays an important regulatory role in the immune system.  CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells.  Mutations in the CTLA-4 gene have been associated with various autoimmune diseases,such as systemic lupus erythematosus,insulin-dependent diabetes mellitus,and other autoimmune diseases.