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Uncoated Human Ep-CAM/CD326 (Epithelial Cell Adhesion Molecule) ELISA Kit (E-UNEL-H0324)

All Size Price Qty
96T*5 $ 399.00
- +
96T*15 $ 958.00
- +
Ancillary Products Available: Ancillary Reagent Kit
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For research use only.

Product Summary

The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range 3.13-200 pg/mL
Sample Volume 100 μL
Total Assay Time 24 h
Reactivity Human
Specificity This kit recognizes Human Ep-CAM/CD326 in samples.No significant cross-reactivity or interference between Human Ep-CAM/CD326 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T*5 / 96T*15
Storage -20℃
Expiration Date 12 months
Typical data
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
pg/mL OD1 OD2 Mean OD Corrected OD
200 2.323 2.289 2.306 2.214
100 1.423 1.375 1.399 1.307
50 0.812 0.772 0.792 0.7
25 0.469 0.471 0.470 0.378
12.5 0.287 0.303 0.295 0.203
6.25 0.184 0.180 0.182 0.09
3.13 0.141 0.141 0.141 0.049
0 0.095 0.089 0.092 -
This ELISA kit uses the Sandwich-ELISA principle. Prior to the assay, the plate must be coated with antibody specific for Human Ep-CAM/CD326. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ep-CAM/CD326 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ep-CAM/CD326, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Ep-CAM/CD326. You can calculate the concentration of Human Ep-CAM/CD326 in the samples by comparing the OD of the samples to the standard curve.
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5; MYC and cyclins A and E.
Uniport ID CAA32870.1
Research Area Cancer , Tags&CellMarkers
Cat.No. Product Name Sizes
E-ELIR-K001 Ancillary Reagent Kit 96T*5
Uncoated Human Ep-CAM/CD326 (Epithelial Cell Adhesion Molecule) ELISA Kit - procedures
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