Uncoated Human IL-15 ( Interleukin 15 ) ELISA Kit (E-UNEL-H0387)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-15 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-15 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IL-15 . You can calculate the concentration of IL-15 in the samples by comparing the OD of the samples to the standard curve.

IL-15 is a regulatory cytokine,and it is produced by dendritic cells,epithelial cells,fibroblasts,and monocytes.   IL-15 plays an important role in immune response and shares many functions with IL-2.   For example,it stimulates the proliferation of activated T cells,NK cells,and B cells,and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand.   In addition,IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages.   IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system.